Improved spectrophotometric assay for lytic polysaccharide monooxygenase
نویسندگان
چکیده
منابع مشابه
A fast and sensitive activity assay for lytic polysaccharide monooxygenase
Background Lytic polysaccharide monooxygenases (LPMO) release a spectrum of cleavage products from their polymeric substrates cellulose, hemicellulose, or chitin. The correct identification and quantitation of these released products is the basis of MS/HPLC-based detection methods for LPMO activity. The duration, effort, and intricate analysis allow only specialized laboratories to measure LPMO...
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other Rhizobium species. Most of these plasmids encoding for fixation and nodulation properties are non-self transmissible. Plasmid profiles were determined for 24 strains of R. trifolii. All the strains harboured at least three plasmids and as many as eight were present in some strains. Southern hybridization with cloned R. meliloti nifgenes was used to identify indigenous Nif plasmids. Each s...
متن کاملFungal cellulose degradation by oxidative enzymes: from dysfunctional GH61 family to powerful lytic polysaccharide monooxygenase family
Our understanding of fungal cellulose degradation has shifted dramatically in the past few years with the characterization of a new class of secreted enzymes, the lytic polysaccharide monooxygenases (LPMO). After a period of intense research covering structural, biochemical, theoretical and evolutionary aspects, we have a picture of them as wedge-like copper-dependent metalloenzymes that on red...
متن کاملActive-site copper reduction promotes substrate binding of fungal lytic polysaccharide monooxygenase and reduces stability
Lytic polysaccharide monooxygenases (LPMOs) are a class of copper-containing enzymes that oxidatively degrade insoluble plant polysaccharides and soluble oligosaccharides. Upon reductive activation, they cleave the substrate and promote biomass degradation by hydrolytic enzymes. In this study, we employed LPMO9C from Neurospora crassa, which is active toward cellulose and soluble β-glucans, to ...
متن کاملStructure of a Thermobifida fusca lytic polysaccharide monooxygenase and mutagenesis of key residues
Background Auxiliary activity (AA) enzymes are produced by numerous bacterial and fungal species to assist in the degradation of biomass. These enzymes are abundant but have yet to be fully characterized. Here, we report the X-ray structure of Thermobifida fusca AA10A (TfAA10A), investigate mutational characterization of key surface residues near its active site, and explore the importance of t...
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ژورنال
عنوان ژورنال: Biotechnology for Biofuels
سال: 2019
ISSN: 1754-6834
DOI: 10.1186/s13068-019-1624-3